Low-Abundance Proteome Discovery: State of the Art and Protocols
Author(s): Dr. Egisto Boschetti (Author), Pier Giorgio Righetti
Publisher: Elsevier
Publication Date: 13 May 2013
Edition: Illustrated
Language: English
Print length: 364 pages
ISBN-10: 0124017347
ISBN-13: 9780124017344
Book Description
Low-Abundance Proteome Discovery addresses the most critical challenge in biomarker discovery and progress: the identification of low-abundance proteins. The book describes an original strategy developed by the authors that permits the detection of protein species typically found in very low abundance and that may yield valuable clues to future discoveries. Known as combinatorial peptide ligand libraries, these new methodologies are one of the hottest topics related to the study of proteomics and have applications in medical diagnostics, food quality, and plant analysis. The book is written for university and industry scientists starting proteomic studies of complex matrices (e.g, biological fluids, biopsies, recalcitrant plant tissues, foodstuff, and beverage analysis), researchers doing wet chemistry, and graduate-level students in the areas of analytical and biochemistry, biology, and genetics.
Editorial Reviews
Review
“The book was written by the inventors of and leading experts in the technology and provides a comprehensive overview of what has been achieved with this relatively new approach. The detailed description of important experimental parameters and the protocols provided in this book make it a unique resource for all practitioners in the field and all scientists interested in the technology.” —Analytical & Bioanalytical Chemistry, February 2014
“Boschetti and Righetti introduce the new technique of combinatorial peptide ligand libraries for identifying low-abundance peptide fragments. The standard proteomics techniques of mass spectrometry, 2D electrophoresis and chromatography are deliberately given brief treatment only as prefractionation tools in favor of new methodologies.” —Reference & Research Book News, October 2013
Review
This book addresses the biggest challenge in the field of protein biomarkers – detecting low-abundance proteomes – and offers novel methods and protocols for their detection, identification, and quantification.
From the Back Cover
Low-Abundance Proteome Discovery: State of the Art and Protocols addresses the biggest challenge in the field of protein biomarkers–the detection of low-abundance proteomes–by using an original strategy developed by the authors. This strategy, known as combinatorial peptide ligand libraries, permits the detection of species of proteins that are typically present in very low abundance and that may yield valuable clues to future discoveries. Low-Abundance Proteome Discovery: State of the Art and Protocols first presents an overview of low-abundance proteins and their importance for research and diagnostics, followed by a survey of conventional prefractionation methods in proteome analysis. Then, it describes the principles and applications of peptide ligand libraries as a unique approach for the detection of low-abundance proteins. Finally, it offers a detailed description of laboratory practices and recipes that will help you succeed in detecting and identifying low-abundance proteins in various situations.
Key features:
Covers novel methodologies for enhancing the visibility of low-abundance proteins
Includes detailed protocols for the detection, identification, and quantification of low-abundance proteins that address real-life needs in laboratory practice
Addresses all applications, including human disease, food and beverage safety, and the discovery of new proteins/peptides of importance in nutraceuticals
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