
Stem Cell Transcriptional Networks: Methods and Protocols
Author(s): Benjamin L Kidder
- Publisher: Humana Press
- Publication Date: April 18, 2014
- Language: English
- Print length: 298 pages
- ISBN-10: 1493905139
- ISBN-13: 9781493905133
Book Description
“Stem Cell Transcriptional Methods and Protocols” collects techniques used to increase our understanding of the underlying transcriptional programs of stem cells that promote self-renewal and differentiation. The volume opens with a section on next-generation sequencing library preparation and data analysis. Continuing with a collection of protocols on visual analysis and interpretation of large-scale interaction networks, this detailed compilation features transcriptional networks in embryonic and adult stem cells, embryo culture and derivation of stem cells, as well as transcriptional programs that promote self-renewal, reprogramming, and transdifferentiation. Written for the highly successful “Methods in Molecular Biology” series, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible protocols and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, “Stem Cell Transcriptional Methods and Protocols” aims to provide a key resource for biologists seeking to interrogate these vital networks.
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