Wow! eBook
A Practical Guide to Protein and Peptide Purification for Microsequencing 2nd Edition
Author(s): Paul T. Matsudaira
- Publisher: Academic Press
- Publication Date: December 2, 2012
- Edition: 2nd
- Language: English
- Print length: 184 pages
- ISBN-10: B00BCR0EWO
- ISBN-13: 9780080924618
Book Description
Why a Second Edition?The Second Edition provides practical answers to the general question, “How can I obtain useful sequence information from my protein or peptide?” rather than the more specific question asked in the first edition, “How can I obtain the N-terminal sequence?” Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry.
– Mass spectrophotometric characterization of proteins and peptides
– Mass spectrophotometric characterization of proteins and peptides
– N-terminal sequencing of proteins with blocked N termini
– Internal amino acid sequence analysis after protease digestion in-gel and on-blot
– Improved microscale peptide purification methods
– Computer analysis of protein sequences
– New protocols tested and refined through everyday use in authors’ laboratories
– Updated reference chapter covering all aspects of protein microsequencing
