Wow! eBook
A Practical Guide to Protein and Peptide Purification for Microsequencing 2nd Edition
Author(s): Paul T. Matsudaira
- Publisher: Academic Press
- Publication Date: 2 Dec. 2012
- Edition: 2nd
- Language: English
- Print length: 184 pages
- ISBN-10: B00BCR0EWO
- ISBN-13: 9780080924618
Book Description
Why a Second Edition?The Second Edition provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide?" rather than the more specific question asked in the first edition, "How can I obtain the N-terminal sequence?" Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry.
- Mass spectrophotometric characterization of proteins and peptides
- Mass spectrophotometric characterization of proteins and peptides
- N-terminal sequencing of proteins with blocked N termini
- Internal amino acid sequence analysis after protease digestion in-gel and on-blot
- Improved microscale peptide purification methods
- Computer analysis of protein sequences
- New protocols tested and refined through everyday use in authors' laboratories
- Updated reference chapter covering all aspects of protein microsequencing
Editorial Reviews
Review
Reviews of the First Edition:
"Like the evening network television news programs, this book gives a useful summary of the highlights (of protein chemistry) to enable the masses to go forth armed with enough information to survive in the world." --Cell
"Like the evening network television news programs, this book gives a useful summary of the highlights (of protein chemistry) to enable the masses to go forth armed with enough information to survive in the world." --Cell
From the Back Cover
Why a Second Edition?
The second edition provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide?" rather than the more specific question asked in the first edition, "How can I obtain the N-terminal sequence?" Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry.
@introbul:Highlights of the Second Edition:
@bul:* Mass spectrophotometric characterization of proteins and peptides
* N-terminal sequencing of proteins with blocked N termini
* Internal amino acid sequence analysis after protease digestion in-gel and on-blot
* Improved microscale peptide purification methods
* Computer analysis of protein sequences
* New protocols tested and refined through everyday use in authors' laboratories
* Updated reference chapter covering all aspects of protein microsequencing
The second edition provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide?" rather than the more specific question asked in the first edition, "How can I obtain the N-terminal sequence?" Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry.
@introbul:Highlights of the Second Edition:
@bul:* Mass spectrophotometric characterization of proteins and peptides
* N-terminal sequencing of proteins with blocked N termini
* Internal amino acid sequence analysis after protease digestion in-gel and on-blot
* Improved microscale peptide purification methods
* Computer analysis of protein sequences
* New protocols tested and refined through everyday use in authors' laboratories
* Updated reference chapter covering all aspects of protein microsequencing
