Author(s): Tanja Zigova (Author, Editor), J. Sanchez (Author, Editor), Paul R. Sanberg (Editor)
Publisher: Humana Press Inc.
Publication Date: 28 Feb. 2002
Language: English
Print length: 400 pages
ISBN-10: 0896039641
ISBN-13: 9780896039643
Book Description
In “Neural Stem Cells: Methods and Protocols”, internationally recognized experts from academic, clinical, and pharmaceutical laboratories describe in great detail the most frequently used cellular, molecular, and electrophysiological methods to isolate, characterize, and utilize neural stem cells. These readily reproducible techniques introduce the various sources of stem/progenitor cells, provide a wide range of conditions for their culture, and make it possible to define their properties in culture. Additional techniques are designed to help researchers identify endogenous stem cells as well as exogenous stem cells after transplantation in the brain. The protocols range from the simplest methods of isolation and characterization of neural cell properties to such very sophisticated methods as characterizing gene expression, telomerase assays, and cell cycle kinetics.
Editorial Reviews
Review
…the book will be of value to stem cell researchers, as the protocols are presented in sufficient detail to make the replication of results likely between laboratories. Neural Stem Cells is a competently collected and presented set of methods. – Cell Therapy News
This detailed volume explores techniques for the study of neurogenic niches and neural lineage progression, specifically addressing major methodological challenges in studying neural stem cells (NSCs) and related cell populations, such as astrocytes, oligodendrocytes, and neurons. The chapters are organized to offer a comprehensive technical foundation, focusing on isolation and identification, monitoring, manipulation, analysis, and the exploration of potential therapeutic applications of NSCs. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Neural Stem Cells: Methods and Protocols serves as an ideal guide for the scientific community and a broader audience interested in understanding neurogenic niches and the progression of neural lineages.
Editorial Reviews
From the Back Cover
This detailed volume explores techniques for the study of neurogenic niches and neural lineage progression, specifically addressing major methodological challenges in studying neural stem cells (NSCs) and related cell populations, such as astrocytes, oligodendrocytes, and neurons. The chapters are organized to offer a comprehensive technical foundation, focusing on isolation and identification, monitoring, manipulation, analysis, and the exploration of potential therapeutic applications of NSCs. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Neural Stem Cells: Methods and Protocols serves as an ideal guide for the scientific community and a broader audience interested in understanding neurogenic niches and the progression of neural lineages.
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