Microbial Plant Pathogens-Detection and Disease Diagnosis:: Viral and Viroid Pathogens, Vol.3 2011th Edition
Author(s): P. Narayanasamy (Author)
Publisher: Springer
Publication Date: November 6, 2014
Edition: 2011th
Language: English
Print length: 342 pages
ISBN-10: 9400789785
ISBN-13: 9789400789784
Book Description
The need for the development of techniques based on the characteristics of the viral proteins and genomic nucleic acids was realized in order to detect, identify, differentiate and quantify viruses in the infected plants/planting materials with or without symptoms of infection. Immunoassays have been successfully applied for the detection of viruses in crop and weed host plant species as well as in the vectors. Nucleic acid-based techniques have been demonstrated to be the most reliable and sensitive tests for detection, identification and differentiation of viruses and viroids present in plants and planting materials.. Inclusion of numerous protocols in appropriate chapters as appendix is a unique feature of this volume.
Editorial Reviews
From the Back Cover
Viruses, with the primitive structural features are capable of causing diseases in all life forms on the earth. With limited morphological characteristics of the virus particles and lack of physiological functions, the need for the development of techniques based on the characteristics of the viral proteins and genomic nucleic acids was realized in order to detect, identify, differentiate and quantify viruses in the infected plants/planting materials with or without symptoms of infection. Immunoassays based on the antigenic properties of viral capsid proteins have been successfully applied for the detection of viruses in the symptomatic and asymptomatic crop and weed host plant species as well as in the vectors that are involved in the transmission of the viruses infecting crop plants. Development of monoclonal antibody technology has enhanced the sensitivity and specificity of immunoassays especially applied for the differentiation of strains of viruses with different virulence levels. Nucleic acid-based techniques have been demonstrated to have higher levels of sensitivity, specificity, reliability, reproducibility and rapidity. The viroids with only an RNA molecule have been detected by techniques based on the biological characteristics in certain cases. Application of immunoassays for viroid detection is not feasible because of the absence of a protein component that is present in the viruses. Nucleic acid-based techniques are the most reliable and sensitive tests for detection, identification and differentiation of viroids present in plants and planting materials. With the aim of providing a comprehensive knowledge of detection of viruses and viroids and diagnosis of the diseases caused by them, information distilled from extensive literature search is presented to fulfil the requirements of the researchers, teachers and graduate students of various disciplines of biological sciences. Inclusion of numerous protocols in appropriate chapters as appendix is a unique feature of this volume to enable the scientists to select the suitable methods for the various investigations.
Microbial Plant Pathogens-Detection and Disease Diagnosis:: Viral and Viroid Pathogens, Vol.3 2011th Edition
Author(s): P. Narayanasamy (Author)
Publisher: Springer
Publication Date: November 3, 2010
Edition: 2011th
Language: English
Print length: 342 pages
ISBN-10: 9048197538
ISBN-13: 9789048197538
Book Description
The need for the development of techniques based on the characteristics of the viral proteins and genomic nucleic acids was realized in order to detect, identify, differentiate and quantify viruses in the infected plants/planting materials with or without symptoms of infection. Immunoassays have been successfully applied for the detection of viruses in crop and weed host plant species as well as in the vectors. Nucleic acid-based techniques have been demonstrated to be the most reliable and sensitive tests for detection, identification and differentiation of viruses and viroids present in plants and planting materials.. Inclusion of numerous protocols in appropriate chapters as appendix is a unique feature of this volume.
Editorial Reviews
From the Back Cover
Viruses, with the primitive structural features are capable of causing diseases in all life forms on the earth. With limited morphological characteristics of the virus particles and lack of physiological functions, the need for the development of techniques based on the characteristics of the viral proteins and genomic nucleic acids was realized in order to detect, identify, differentiate and quantify viruses in the infected plants/planting materials with or without symptoms of infection. Immunoassays based on the antigenic properties of viral capsid proteins have been successfully applied for the detection of viruses in the symptomatic and asymptomatic crop and weed host plant species as well as in the vectors that are involved in the transmission of the viruses infecting crop plants. Development of monoclonal antibody technology has enhanced the sensitivity and specificity of immunoassays especially applied for the differentiation of strains of viruses with different virulence levels. Nucleic acid-based techniques have been demonstrated to have higher levels of sensitivity, specificity, reliability, reproducibility and rapidity. The viroids with only an RNA molecule have been detected by techniques based on the biological characteristics in certain cases. Application of immunoassays for viroid detection is not feasible because of the absence of a protein component that is present in the viruses. Nucleic acid-based techniques are the most reliable and sensitive tests for detection, identification and differentiation of viroids present in plants and planting materials. With the aim of providing a comprehensive knowledge of detection of viruses and viroids and diagnosis of the diseases caused by them, information distilled from extensive literature search is presented to fulfil the requirements of the researchers, teachers and graduate students of various disciplines of biological sciences. Inclusion of numerous protocols in appropriate chapters as appendix is a unique feature of this volume to enable the scientists to select the suitable methods for the various investigations.
Morphological, biological, biochemical and physiological characteristics have been used for the detection, identification and differentiation of fungal pathogens up to species level. Tests based on biological characteristics are less consistent. Immunoassays have been shown to be effective in detecting fungal pathogens present in plants and environmental samples. Development of monoclonal antibody technology has greatly enhanced the sensitivity and specificity of detection, identification and differentiation of fungal species and varieties/strains. Nucleic acid-based techniques involving hybridization with or amplification of unique DNA have provided results rapidly and reliably. Presentation of a large number of protocols is a unique feature of this volume.
Editorial Reviews
Review
From the reviews:
“The first volume of the Microbial plant pathogens – Detection and disease diagnosis focuses on fungal pathogens. … All chapters are summarized in the abstract, well referenced and focused on providing concerns of techniques and improvements of research. Therefore, if you are interested in the methods for detection and diagnosis, this is certainly a book you must have. The book should be available to every mycologist and students studying any aspect of plant pathology. Every library in the world should also have this book.” (Dhanushka Udayanga and Dimuthu S. Manamgoda, Fungal Diversity, April, 2012)
From the Back Cover
Plants are infected by different microbial pathogens, of which fungal pathogens form the highly evolved and earliest recognized group. The morphological, biological, biochemical and physiological characteristics have been used for the detection, identification and differentiation of fungal pathogens up to species level. Pathogenicity on a set of differential host plant species or crop cultivars has been useful for the identification of physiologic races existing within a morphologic species/variety. As the techniques based on the above mentioned characteristics require long time and yield inconsistent results, the need for the development of techniques with greater accuracy, reliability, reproducibility and capable of providing the results rapidly was realized. Immunoassays have been shown to be effective in detecting several fungal pathogens present in plants, planting materials, soil, water and air. Development of monoclonal antibody technology has greatly enhanced the sensitivity and specificity of detection, identification and differentiation of fungal species and varieties/strains. Nucleic acid-based techniques involving hybridization of probes with complementary sequences of the target pathogen(s) or amplification of specific sequences of the target pathogen DNA/fragment have been demonstrated to be the most efficient among the diagnostic techniques currently available. This volume provides exhaustive information on various methods of detection of fungal pathogens and the diagnosis of the diseases caused by them based on extensive literature search that will be useful to the researchers, teachers and graduate students of different disciplines of biological sciences, in addition to the extension pathologists, personnel of plant quarantines and certification programs. Presentation of a large number of protocols is a unique feature of this volume to facilitate selection of appropriate techniques for different investigations.
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