Culture of Human Stem Cells

Culture of Human Stem Cells book cover

Culture of Human Stem Cells

Author(s): R. Ian Freshney (Author), Glyn N. Stacey (Author), Jonathan M. Auerbach (Author)

  • Publisher: Wiley-Liss
  • Publication Date: June 22, 2007
  • Edition: 1st
  • Language: English
  • Print length: 360 pages
  • ISBN-10: 0470052465
  • ISBN-13: 9780470052464

Book Description

This book collects the most effective and cutting-edge methods and protocols for deriving and culturing human embryonic and adult stem cells―in one handy resource.

This groundbreaking book follows the tradition of previous books in the Culture of Specialized Cells Series―each methods and protocols chapter is laid out exactly like the next, with stepwise protocols, preceded by specific requirements for that protocol, and a concise discussion of methods illustrated by data. The editors describe a limited number of representative techniques across a wide spectrum of stem cells from embryonic, newborn, and adult tissue, yielding an all-encompassing and versatile guide to the field of stem cell biology and culture.

The book includes a comprehensive list of suppliers for all equipment used in the protocols presented, with websites available in an appendix. Additionally, there is a chapter on quality control, and other chapters covering legal and ethical issues, cryopreservation, and feeder layer culture. This text is a one-stop resource for all researchers, clinical scientists, teachers, and students involved in this crucial area of study.

Editorial Reviews

Review

“For those working, or considering working with human stem cells, Culture of Human Stem Cells will be an essential reference.” (The Biochemical Society, October 2009)

“Valuable to those entering the field from a wide spectrum of disciplines…an essential textbook for teachers and students who are involved with the therapeutic potential of stem cell research.” (SirReadaLot.org, October 2007)

“The principal value of this book lies in the diversity of topics. In addition to chapters on embryonic stem (ES) cells, interesting chapters cover the derivation of multipotential stem cells from mammary tissue, dental pulp, cornea, cord blood, and adipose tissue.” (Doody’s)

From the Inside Flap

The first book to collect the most effective and cutting-edge methods and protocols for deriving and culturing human embryonic and adult stem cells—in one handy resource

This groundbreaking new book follows the tradition of previous books in the Culture of Specialized Cells series—each methods and protocols chapter is laid out exactly like the next, with stepwise protocols, preceded by specific requirements for that protocol, and a concise discussion of methods illustrated by data. The editors describe a limited number of representative techniques across a wide spectrum of stem cells from embryonic, newborn, and adult tissue, yielding an all-encompassing and versatile guide to the field of stem cell biology and culture.

Culture of Human Stem Cells includes a comprehensive list of suppliers for all equipment used in the protocols presented, with Web sites available in an appendix. Additionally, there is a chapter on quality control, and other chapters covering legal and ethical issues, cryopreservation, and feeder layer culture. Culture of Human Stem Cells is a one-stop resource for all researchers, clinical scientists, teachers, and students involved in this crucial area of study.

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