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Cell Separation: A Practical Approach
Author(s): Derek Fisher (Editor), Gillian E. Francis (Editor), David Rickwood (Editor)
- Publisher: Oxford University Press
- Publication Date: February 25, 1999
- Edition: 1st
- Language: English
- Print length: 286 pages
- ISBN-10: 019963579X
- ISBN-13: 9780199635795
Book Description
The first chapter describes the isolation of single cell suspensions from animal tissues and from adherent cell culture. It includes the use of lavages, problems of extracellular matrix, methods of assessing cell viability, and the separation of viable from non-viable cells. The following seven chapters discuss specific methods for the separation of cells and the fractionation of cell populations. Sedimentation methods, centrifugal elutriation, partitioning in aqueous two-phase systems, flow cytometry, as well as numerous other procedures are discusses as well.
This book should be read by all researchers for whom cell separation is the starting point for their experimental work. As such, it is an invaluable laboratory manual for cell biologists, biochemists, molecular biologists, and physiologists.
Editorial Reviews
Review
"The title of this book describes the contents within, and reviews a subject that is underrepresented in the literature. The seven chapters discuss the preparation of single cell suspensions and six typical separation methods: sedimentation, centrifugal elutriation, partitioning in aqueous two-phase systems, flow cytometry, immunomethods (magnetic, column, and panning techniques), and free flow electrophoresis. The descriptions emphasize practical applications of these techniques including protocols for most, if not all methods. Although the theoretical basis of these techniques are not heavily emphasized, adequate references are given for those interested."--The Quarterly Review of Biology
