Bacterial Cellular Metabolic Systems: Metabolic Regulation of a Cell System with 13C-metabolic Flux Analysis

Bacterial cellular metabolic systems focuses on this highly topical subject in relation to culture environment and provides a detailed analysis from gene level to metabolic level regulation, as well as offering a discussion of the most recent modelling approaches. The book begins with an introduction to metabolic mechanisms and to the metabolic regulation of a cell, before moving on to discussing the action of global regulators in response to a specific culture environment. The second half of the book examines conventional flux balance analysis and its applications, 13C-metabolic flux analysis, and the effect of a specific gene knockout on the metabolism.

Key Features: comprehensive account of metabolic regulation via global regulators in response to changes in the culture environment; basic formulation of 13C-metabolic flux analysis based on 13C-labelling experiments; systems biology approach for the modelling and computer simulation of the main metabolic pathways of a cell system.

Contents: Main metabolism; Brief overview of metabolic regulation of a bacterial cell; Metabolic regulation by global regulators in response to culture environment; Conventional flux balance analysis and its applications; 13C-metabolic flux analysis and its applications; Effect of a specific-gene knockout on metabolism.