The complexity and heterogeneity of biological systems has posed an immense challenge in recent years. An increasingly important tool for obtaining molecular and atomic scale information on a range of large biological molecules and cellular components is solid state NMR. This technique can address fascinating problems in structural biology, including the arrangement of supramolecular complexes and fibril formation in relation to molecular folding, misfolding and aggregation.

Advances in Biological NMR brings the reader up to date with chapters from international leaders of this growing field, covering the most recent developments in the methodology and applications of solid state NMR to studies of membrane interactions and molecular motions. A much needed discussion of membrane systems is detailed alongside important developments in in situ analysis.

Topics include applications to biological membranes, membrane active peptides, membrane proteins, protein assemblies and in-cell NMR. This exposition of an invaluable technique will interest those working in a range of related spectroscopic and biological fields. A basic introduction invites those interested to familiarise themselves with the basic mathematical and conceptual foundations of solid state NMR. A thorough and comprehensive discussion of this promising technique follows, which is essential reading for those working or studying at postgraduate level in this exciting field.

Advances in Biological Solid-State NMR

Proteins and Membrane-Active Peptides

By Frances Separovic, Akira Naito

The Royal Society of Chemistry

Copyright © 2014 The Royal Society of Chemistry
All rights reserved.
ISBN: 978-1-84973-910-8

Contents

Chapter 1 Introduction to Biological Solid-State NMR M. Weingarth and M. Baldus,
Chapter 2 Combining NMR Spectroscopic Measurements and Molecular Dynamics Simulations to Determine the Orientation of Amphipathic Peptides in Lipid Bilayers B. Scott Perrin Jr., Richard W. Pastor and Myriam Cotten,
Chapter 3 13C–13C Distance Measurements by Polarization Transfer Matrix Analysis of 13C Spin Diffusion in a Uniformly 13C-Labeled Molecular Complex under Magic Angle Spinning Ayako Egawa, Hideo Akutsu and Toshimichi Fujiwara,
Chapter 4 Demonstration of the Equivalence of Solid-State NMR Orientational Constraints from Magnetic and Rotational Alignment of the Coat Protein in a Filamentous Bacteriophage Stanley J. Opella and Bibhuti B. Das,
Chapter 5 Membrane Protein Interactions James A. Jarvis and Philip T. F. Williamson,
Chapter 6 Magnetic Liposomes and Bicelles: New Tools for Membrane-Peptide Structural Studies Erick J. Dufourc, Nicole Harmouche, Cécile Loudet-Courrèges, Reiko Oda, Anna Diller, Benoit Odaert, Axelle Grélard and Sébastien Buchoux,
Chapter 7 Membranes and Their Lipids: A Molecular Insight into Their Organization and Function Martin Lidman, Marcus Wallgren and Gerhard Gröbner,
Chapter 8 Structural Studies of Small Bioactive Compounds Interacting with Membranes and Proteins Shigeru Matsuoka and Michio Murata,
Chapter 9 Lipopolysaccharide Induces Raft Domain Expansion in a Cholesterol-Containing Membrane Kaoru Nomura and Shoichi Kusumoto,
Chapter 10 Deuterium NMR of Mixed Lipid Membranes Sherry S. W. Leung and Jenifer Thewalt,
Chapter 11 Membrane Interactions of Amphiphilic Peptides with Antimicrobial Potential: A Solid-State NMR Study Matthieu Fillion, Normand Voyer and Michèle Auger,
Chapter 12 Investigations of the Structure, Topology and Dynamics of Membrane-Associated Polypeptides by Solid-State NMR Spectroscopy Evgeniy S. Salnikov, Christopher Aisenbrey, Jesus Raya and Burkhard Bechinger,
Chapter 13 NMR of Lipids and Lipid/Peptide Mixtures James H. Davis, Miranda L. Schmidt and Ivana Komljenovic,
Chapter 14 NMR Investigations of the Structure and Dynamics of Antimicrobial Peptides: The Peptaibol Alamethicin Thomas Vosegaard and Niels Chr. Nielsen,
Chapter 15 Solid-State NMR Studies of Antimicrobial Peptide Interactions with Specific Lipid Environments Marc-Antoine Sani and Frances Separovic,
Chapter 16 Dynamic Structure Analysis of Peptides in Membranes by Solid-State NMR Erik Strandberg and Anne S. Ulrich,
Chapter 17 Structural Dynamics of Retinal in Rhodopsin Activation Viewed by Solid-State 2H NMR Spectroscopy Michael F. Brown and Andrey V. Struts,
Chapter 18 Helical Membrane Protein Structure: Strategy for Success Nabanita Das, Dylan T. Murray, Yimin Miao and Timothy A. Cross,
Chapter 19 Chemistry and Structure via Solid-State NMR Judith Herzfeld,
Chapter 20 Photoactivated Structural Changes in Photoreceptor Membrane Proteins as Revealed by in situ Photoirradiation Solid-State NMR Spectroscopy Akira Naito and Izuru Kawamura,
Chapter 21 A Promising Prognosis for Solid-State NMR of Functional Membrane Protein Complexes Michael J. Harris and Lynmarie K. Thompson,
Chapter 22 Structural Topologies of Phosphorylated and Non-phosphorylated Oligomeric Phospholamban in Lipid Membranes by a Hybrid NMR Approach Vitaly Vostrikov and Gi