RNA Folding:Methods and Protocols (Methods in Molecular Biology, 2726)

by: Ronny Lorenz (Editor)

Publisher: Humana

Edition: 2024th

Publication Date: 2024/5/24

Language: English

Print Length: 414 pages

ISBN-10: 1071635182

ISBN-13: 9781071635186

Book Description

To understand and predict the behavior and function of RNAs, sophisticated tools are required to simulate and analyze their potential for forming structures. This volume discusses the various levels of prediction and algorithmic approaches to RNA folding. The chapters in this book cover topics such as energy parameters of the nearest-neighbor (NN) energy model; classified dynamic programming to address exponential growth of candidate structures that an RNA molecule may fold into; sequence evolution and conserved structures among multiple RNA sequences; the latest framework capable of handling both positive and negative RNA sequence design objectives; and kinetic folding approaches that look at the dynamic nature of RNA folding. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, RNA Folding:Methods and Protocols is a valuable resource for researchers who are interested in leaing more about this important and developing field.

About the Author

To understand and predict the behavior and function of RNAs, sophisticated tools are required to simulate and analyze their potential for forming structures. This volume discusses the various levels of prediction and algorithmic approaches to RNA folding. The chapters in this book cover topics such as energy parameters of the nearest-neighbor (NN) energy model; classified dynamic programming to address exponential growth of candidate structures that an RNA molecule may fold into; sequence evolution and conserved structures among multiple RNA sequences; the latest framework capable of handling both positive and negative RNA sequence design objectives; and kinetic folding approaches that look at the dynamic nature of RNA folding. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, RNA Folding:Methods and Protocols is a valuable resource for researchers who are interested in leaing more about this important and developing field.