Plant Stress Tolerance: Methods and Protocols (Methods in Molecular Biology, 2832)
by: Ramanjulu Sunkar (Editor)
Publisher: Humana
Publication Date: 2024/6/14
Language: English
Print Length: 304 pages
ISBN-10: 1071639722
ISBN-13: 9781071639726
Book Description
This fully updated new edition explores new techniques for studying plant stress. This includes novel methodologies such as MeRIP-seq for identifying changes in m6A profiles, isolation of stress granules, and additional methodologies such as MNase–seq for identifying nucleosome occupancy, alteative splicing analysis, identifying proteins that interact with long noncoding RNAs, untargeted metabolomics, ROS and NO measurements, priming-related protocols, growth-promoting bacteria isolation and functional characterization, as well as isolating mutants for stress-regulated genes using CRISPR technology. Written for the highly successful Methods in Molecular Biology series, chapters feature introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and up-to-date, Plant Stress Tolerance: Methods and Protocols, Third Edition provides a wide range of protocols catering to the needs of plant physiologists, biochemists, and molecular biologists interested in probing this vital area of study.
About the Author
This fully updated new edition explores new techniques for studying plant stress. This includes novel methodologies such as MeRIP-seq for identifying changes in m6A profiles, isolation of stress granules, and additional methodologies such as MNase–seq for identifying nucleosome occupancy, alteative splicing analysis, identifying proteins that interact with long noncoding RNAs, untargeted metabolomics, ROS and NO measurements, priming-related protocols, growth-promoting bacteria isolation and functional characterization, as well as isolating mutants for stress-regulated genes using CRISPR technology. Written for the highly successful Methods in Molecular Biology series, chapters feature introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and up-to-date, Plant Stress Tolerance: Methods and Protocols, Third Edition provides a wide range of protocols catering to the needs of plant physiologists, biochemists, and molecular biologists interested in probing this vital area of study.