KRAS:Methods and Protocols:2797 (Methods in Molecular Biology, 2797)

by: Andrew G. Stephen (Editor),Dominic Esposito(Editor)

Publisher: Humana

Publication Date: 4 April 2024

Language: English

Print Length: 376 pages

ISBN-10: 1071638211

ISBN-13: 9781071638217

Book Description

This volume details protocols ranging from high yield production metabolically labeled KRAS for NMR studies to approaches that quantify engagement of novel molecules that bind KRAS in live cells. Chapters focus on protein production and characterization, biochemical assays, cell-based assays, KRAS-membrane interactions, targeting KRAS, and cell models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, KRAS:Methods and Protocols aims to provide methods that will be instrumental in the development of future clinically approved KRAS therapeutics.