Genetically Incorporated Non-Canonical Amino Acids:Methods and Protocols:2676 (Methods in Molecular Biology, 2676)

by: Yu-Hsuan Tsai (Editor),Simon J. Elsässer(Editor)

Publisher: Humana

Publication Date: 6 Jun. 2023

Language: English

Print Length: 297 pages

ISBN-10: 1071632507

ISBN-13: 9781071632505

Book Description

This detailed volume explores non-canonical amino acids (ncAAs) through their site-specific incorporation by genetic code expansion (GCE). The collection provides a broad resource of methods for implementing GCE in E. coli, mammalian cells, and animals, highlighting specific applications ranging from fluorescence labeling to photocontrol and the study of protein post-translational modification. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Genetically Incorporated Non-Canonical Amino Acids:Methods and Protocols serves as an ideal source of methodologies that can be adapted and extended, migrated to different model systems, and combined in new ways to help explore a wide range of biological questions and to augment industrial and pharmaceutical protein engineering.