Baculovirus:Methods and Protocols (Methods in Molecular Biology, 2829)

by: Manon M.J. Cox (Editor)

Publisher: Humana

Publication Date: 2024/7/3

Language: English

Print Length: 358 pages

ISBN-10: 1071639609

ISBN-13: 9781071639603

Book Description

This detailed volume provides up-to-date guidance on techniques used to work with baculoviruses and insect cells. The book provides basic methods to create recombinant baculoviruses, to improve productivity, to produce a variety of products, to purify products, to quantify baculovirus stocks or to quantify product produced, and it concludes with alteative uses of either baculovirus or insect cells as tools. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and comprehensive, Baculovirus:Methods and Protocols serves as an ideal guide for researchers looking to overcome some of the limitations associated with the early baculoviral vectors and cell lines.

About the Author

This detailed volume provides up-to-date guidance on techniques used to work with baculoviruses and insect cells. The book provides basic methods to create recombinant baculoviruses, to improve productivity, to produce a variety of products, to purify products, to quantify baculovirus stocks or to quantify product produced, and it concludes with alteative uses of either baculovirus or insect cells as tools. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and comprehensive, Baculovirus:Methods and Protocols serves as an ideal guide for researchers looking to overcome some of the limitations associated with the early baculoviral vectors and cell lines.